Optimum conditions for electric pulse-mediated gene transfer to Bacillus subtilis cells.
نویسندگان
چکیده
منابع مشابه
Optimum conditions for electric pulse-mediated gene transfer to mammalian cells in suspension.
A pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells. In the transformation of tk- cells with pTK1, a single pulse of 100-2000 microseconds gave a high transformation frequency at 1.5-6 kV/cm and room temperature, the highest transformation frequency obtained b...
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Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters was systematically investigated using the transient expression of the chloramphenicol acetyltransferase and the beta-galactosidase activities in Chinese hamster ovary cells. Pulsing conditions induc...
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A simple physical chemical method of transferring genes into eucaryotic cells is described. Electric impulses in the intensity range of 5-10 kV/cm with a duration of 5-10 bs were found to appreciably increase the uptake of DNA into cells. After electric field treatment, stable transformants were obtained in a system containing mouse cells deficient in thymidine kinase and a plasmid DNA harbouri...
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Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA. The integration of homologous donor DNA into the recipient chromosome requires RecA, while plasmid establishment, which is independent of RecA, requires at least RecO and RecU. RecA and RecN colocalize at the polar DNA uptake machinery,...
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Optimization of Bacillus subtilis (isolate B246) growth conditions for mass cell production under laboratory conditions was investigated as part of a biological control programme. Aspects such as increasing yield using various starter culture volumes, various media and incubation time and temperatures, were studied. No significant differences between starter culture volumes were found. Potato m...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.53.2441